IMMUNOBIOLOGY Interleukin-9 enhances interleukin-5 receptor expression, differentiation, and survival of human eosinophils

Interleukin-9 (IL-9) has been implicated in the pathogenesis of allergic disorders. To examine the interaction between IL-9 and eosinophils, we evaluated mature peripheral blood eosinophils for their expression of the specific a-subunit of the IL-9 receptor (IL-9R–a). The expression of IL-9R–a by human eosinophils was detected at the messenger RNA (mRNA) and protein levels by reverse transcriptase–polymerase chain reaction (RT-PCR), flow cytometry, and immunocytochemical analysis, respectively. Functional analyses demonstrated that recombinant human (rh)IL-9 inhibited in vitro peripheral blood human eosinophil apoptosis in a concentration-dependent manner. We then examined the role of IL-9 in eosinophil differentiation using the human cord blood CD341 cells and human promyelocytic leukemia cells (HL-60). The addition of IL-9 to CD341 cells cultured in IL-3 and IL-5 enhanced eosinophil development, and IL-9 alone induced the expression of IL5R–a. IL-9 also up-regulated the IL-5R–a chain cell surface expression during terminal eosinophil differentiation of the HL-60 cell line. Our findings suggest that IL-9 may potentiate in vivo eosinophil function by increasing their survival and IL-5– mediated differentiation and maturation. Taken together, these results suggest a mechanism by which IL-9 potentiates airway and tissue eosinophilia. (Blood. 2000; 96:2163-2171)